Depositors Notes: 4C9H4 is an excellent marker for cytokinesis, and labels the cleavage furrow of dividing embryonic and imaginal disc cells. It recognizes the product of the peanut gene, which encodes a protein similar to the bud neck filament proteins of S. cerevisiae (CDC3, CDC10, CDC11 and CDC12).
Predicted Species Reactivity:
Immunogen: GST-fusion protein containing the amino-terminal 116 aa of the peanut protein.
Alternate Gene Names: CG8705
Immunogen Sequence: Partial protein
Isotype: MIgG1, kappa light chain
Alternate Antibody Name: 4C9
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence: amino-terminal 116 aa
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4C9H4 anti-peanut was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 4C9H4 anti-peanut)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.