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AD4.4(EcR-B1)
$40.00


View product citations for antibody AD4.4(EcR-B1) on CiteAb
 
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Antigen: ecdysone receptor (EcR-B1)

Hybridoma Cells Available: Yes
Antigen Species: Drosophila Depositor: Thummel, C. / Hogness, D.
Isotype: MIgG1, kappa light chain Antigen Sequence:
Host Species: mouse Depositors Institution: University of Utah
Positive Tested Species Reactivity: Drosophila, Sciara coprophila Depositors Notes:
Antigen Molecular Weight: Predicted: 94 kDa; Apparent: 105 kDa Human Protein Atlas: Link
Predicted Species Reactivity:   Gene: EcR
Immunogen: TrpE-EcR-B1 fusion protein containing both isoform-specific and common segments of EcR-Bl (residues 68-878). Alternate Gene Names: NR1H1
Alternate Antibody Name: Clonality: Monoclonal
Alternate Antigen Name: Epitope Mapped: Yes
Myeloma Strain: SP2/0 Epitope Location or Sequence: aa 68-222
Uniprot ID: Immunogen Sequence: Partial protein
Entrez Gene ID: Additional Characterization: Link
Antibody Registry ID: AB_2154902 Additional Information:  
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
   
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AD4.4(EcR-B1) was deposited to the DSHB by Thummel, C. / Hogness, D. (DSHB Hybridoma Product AD4.4(EcR-B1))

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ChIP References
  • ELISA References
  • FFPE References
  • FB References
  • FACS References
  • Microarray References
  • Epitope Map References
  • Gel Supershift References
  • All References

Initial Publication

IHC References

IP References

IF References

WB References

ChIP References

ELISA References

FFPE References

FB References

FACS References

Microarray References

Epitope Map References

Gel Supershift References

All References

Developmental Studies Hybridoma Bank
University of Iowa, Department of Biology
028 Biology Building East
Iowa City, Iowa 52242-1324


Phone: 319-335-3826
Secure Fax: 319-359-4079
Email: dshb@uiowa.edu


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