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145AA1.1
$40.00


View product citations for antibody 145AA1.1 on CiteAb
 
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Antigen: Aggregatibacter actinomycetemcomitans serotype b

Hybridoma Cells Available: No
Antigen Species: A. actinomycetemcomitans Depositor: Gmuer, R.
Antigen Sequence: Depositors Institution: Institute of Oral Biology, ZZMK, University of Zuerich
Antigen Molecular Weight: Human Protein Atlas: Link
Positive Tested Species Reactivity: gram-negative bacteria Depositors Notes:
Predicted Species Reactivity:   Gene:
Immunogen: Aggregatibacter actinomycetemcomitans, strain OMZ 250A (= Y4, ATCC 43718), cell-free culture supernatant of 24 h culture in fluid medium Alternate Gene Names:
Immunogen Sequence: Isotype: MIgG2a
Alternate Antibody Name: Clonality: Monoclonal
Alternate Antigen Name: Epitope Mapped: No
Myeloma Strain: Designation of F0 Epitope Location or Sequence:
Uniprot ID: Host Species: mouse
Entrez Gene ID: Additional Characterization: Link
Antibody Registry ID: Additional Information: *Cell lines will be distributed only with written consent from the director of the Institute of Oral Biology, University of Zuerich, Zuerich, Switzerland. Contact the DSHB for details (dshb@uiowa.edu). The immunization of the antibody was in 1986 and used AlK(SO4) precipitate of approximately 100 ml culture supernatant. The route of immunization was intraperitoneal and the adjuvant was 50% incomplete Freund's adjuvant (1st immunization), none (2nd immunization). The origin of the antibody came from J. Slots, Univ. of Pennsylvania, Philadelphia, USA.  
Recommended Applications: Immunofluorescence, Western Blot
   
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
145AA1.1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 145AA1.1)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ChIP References
  • ELISA References
  • FFPE References
  • FB References
  • FACS References
  • Microarray References
  • Epitope Map References
  • Gel Supershift References
  • All References

Initial Publication

IHC References

IP References

IF References

WB References

ChIP References

ELISA References

FFPE References

FB References

FACS References

Microarray References

Epitope Map References

Gel Supershift References

All References

Developmental Studies Hybridoma Bank
University of Iowa, Department of Biology
028 Biology Building East
Iowa City, Iowa 52242-1324


Phone: 319-335-3826
Secure Fax: 319-359-4079
Email: dshb@uiowa.edu


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