Positive Tested Species Reactivity: gram-negative bacteria
Depositors Notes:
Gene:
Immunogen: Aggregatibacter actinomycetemcomitans, strain OMZ 247 (= NCTC 9710 or ATCC 33384), cell-free culture supernatant of 24 h culture in fluid medium
Additional Information: *Cell lines will be distributed only with written consent from the director of the Institute of Oral Biology, University of Zuerich, Zuerich, Switzerland. Contact the DSHB for details (dshb@uiowa.edu). Immunization took the intraperitoneal route and used AlK(SO4) precipitate of approximately 100 ml culture supernatant amount in 1986. THe adjuvant of the antibody during immunization was 50% incomplete Freund's adjuvant (1st immunization), none (2nd immunization).
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
138AA1.1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 138AA1.1)
Storage and Handling Recommendations
Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.
