Depositors Notes: This antibody strongly stains the axons of the CNS with virtually undetectable staining of neuron cell bodies, the PNS, or any other tissues of the embryo. It is an excellent marker for the pattern of commissures and connectives. The antibody can be used to stain dissections without detergent. The antibody probably recognizes a carbohydrate epitope based on its insensitivity to glutaraldehyde and sensitivity to periodate.
Predicted Species Reactivity:
Immunogen: Membrane proteins from cultured embryonic Drosophila neuronal cells.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BP 102 anti-CNS axons was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product BP 102 anti-CNS axons)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.