C566.9
Catalog Fields
Product Name/ID: C566.9
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: cora
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_1161642
Uniprot ID: Q9V8R9
Entrez Gene ID: 37205
Clonality: Monoclonal
Immunogen: Recombinant Coracle FERM domain (aa 72-397)/GST fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: Coracle (common)
Epitope Location or Sequence: aa 72-397 of isoform 1 (Uniprot ID: Q9V8R9-1).
Alternate Antigen Name:
Deposit Date: 8/25/2007
Antigen Molecular Weight: Predicted: multiple isoforms from 78 to 184 kDa. Apparent: 200 kDa.
Depositor: Fehon, R.
Antigen Sequence:
Depositor Institution: University of Chicago
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: This antibody will recognize all coracle isoforms except maybe isoform 5 (Uniprot ID: Q9V8R9-5). This antigen is also known as protein 4.1 homolog and is a component of septate junctions.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C566.9 was deposited to the DSHB by Fehon, R. (DSHB Hybridoma Product C566.9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene.
Artavanis-Tsakonas S
Development (Cambridge, England) 120.3 (1994 Mar): 545-57.