Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human
Depositors Notes: CD11a, as as component of the LFA-1 integrin, is the receptor for ISG15 protein (PMID: 29100055) to signal cytokine secretion. This antibody does recognizes CD11a in acetone-fixed frozen sections but fails in all aldehyde-fixation protocols.
Recommended Applications: FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MHM.24 was deposited to the DSHB by Hildreth, J.E.K. (DSHB Hybridoma Product MHM.24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.