References: J. Biol. Chem. 263(2), 994-1001.; J. Cardiovasc. Pharmacol. 12(Suppl. 4), S86-S90.; Proceedings from the Bayer Centenary Symposium. In: The Calcium Channel: Structure, Function and Implications. (M. Morad, W. Nayler, S. Kazda, M. Schramm, eds.). Springer-Verlag Berlin Heidelberg, Germany, pp. 586-600.; J. Biol. Chem. 264, 2816-2825.; Ann. N.Y. Acad. Sci. 560, 251-257.; J. Cell Biol. 109, 135-147.; J. Cell Biol. 110, 1173-1185.; J. Cell Biol. 112, 135-148.; J. Biol. Chem. 266, 18914-18919.; Science 261, 486-489.; Amer. J. Physiol. Cell Physiol. 267, C282-C292.; Methods Enzymol. 238, 335-348.; J. Biol. Chem. 270, 12056-12064.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
VD2(1)B12 was deposited to the DSHB by Campbell, K.P. (DSHB Hybridoma Product VD2(1)B12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 μg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 μg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.
Developmental Studies Hybridoma Bank
University of Iowa, Department of Biology
028 Biology Building East
Iowa City, Iowa 52242-1324