Additional Information: In the original paper for E1.9 [PMID: 2300585], the immunogen was described as rat E12, but the subsequent paper from the same authors and in the Depositor's notes it was described as mouse E11. The E1.9 epitope is developmentally regulated. E1.9 recognizes a cytoplasmic epitope in the primary sensory and motor neurons during axonal outgrowth, appearing at E8.5 and disappearing by E12 in the mouse when the same cell populations become positive for mAb B10. It stains neural crest-derived sensory neurons (e.g., neurons of dorsal root ganglia) as well as placode-derived sensory neurons (e.g.neurons of cranial nerves VII and VIII). It is not clear which filaments are recognized by this antibody. For this reason we didn't link it to a Uniprot or gene ID. See mAb B10 for a related antibody.
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
E1.9 was deposited to the DSHB by Gilbert, W. / Stainier, D. (DSHB Hybridoma Product E1.9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.