Product Form Descriptions
Supernatant is serum-free hybridoma conditioned culture media containing bovine serum albumin for protein stabilization (HB101 medium).
Concentrate is produced either by ultrafiltration through a 100 kD exclusion filter or by dilution of bioreactor fluid. Concentrate is not a purified product but does contain 10 times the immunoglobulin found in the supernatant. Therefore, 100 μl of concentrate contains the same amount of monoclonal antibody as 1 ml of supernatant. Concentrate is recommended only for protocols requiring high antibody concentration in a small volume (i.e., immunoprecipitation, microinjection or antibody conjugation). For routine staining or blotting protocols, supernatant is recommended.
Ascites is the accumulation of excess fluid due to growth of hybridomas after injection of the cells into the peritoneal space of a mouse. Ascites fluid will contain highly concentrated monoclonal antibody along with other mouse proteins. The Ascites product available from DSHB has been centrifuged and filter sterilized.
Bioreactor Supernatant is produced by sequestering hybridoma cells in a specialized tissue culture apparatus, (bioreactor) with media that allows for the production of highly concentrated supernatant.

ProClin 150
To increase product stability, the antimicrobial agent ProClin 150 is now added to all DSHB products (supernatant, concentrate, biosupe, and ascites fluid).

Product Storage
Although many cell products are maintained at 4°C for years without loss of activity, each antibody is different, and the shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks and 2) the remaining product diluted with an equal volume of molecular biology grade glycerol and stored at -20°C.

Supernatant Use Recommendations
Although the optimal Ig concentration for an application varies for each monoclonal antibody and must be determined empirically, a good starting concentration for immunohistochemistry (IHC) is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml). Immunoglobulin concentration of ascites is generally >1 mg/ml, therefore a starting dilution of ascites for IHC is 1:1000.

Shipping deadlines
US orders: To be sure your order is shipped the same week you order, please place your order by 11 AM Central time on Wednesday. If the order is correct and the items are available, we will do our best to ship for delivery that week.

International orders: Be sure to place your order by 11 AM US Central time on Thursday. If the order is correct and the items are available, we will do our best to ship the following Monday or Tuesday, depending on the country.

US shipping details
Shipping costs are estimates only; actual costs may differ. Shipping charge for domestic overnight delivery of cell products is $20.00 per shipment. Shipment of frozen cells and delivery to extended areas will be higher.

Freight terms are FOB Origin via DSHB-preferred carrier, pre-paid and added to the invoice. Purchase orders should indicate freight terms.

Shipping to South Korea
Due to customs clearance issues, all orders to Korea must be routed through Biomax Co., Ltd. Please contact Jaehoon Kim to determine handling fees and to submit your order.

Payment
The Developmental Studies Hybridoma Bank accepts payment by VISA, MasterCard and American Express, by check in US dollars drawn on a US bank, by international money order or by wire transfer. To pay by credit card, please provide card details with your order including card number, expiration date, cardholder name, and the email address where the payment receipt should be sent. For other payment methods, please include a purchase order number from your institution with your order. For payment of international invoices by wire transfer, our preferred method of payment is through peerTransfer. With peerTransfer, you can:

The Developmental Studies Hybridoma Bank accepts payment by VISA, MasterCard and American Express, by check in US dollars drawn on a US bank, by international money order or by wire transfer. To pay by credit card, please provide card details with your order including card number, expiration date, cardholder name, and the email address where the payment receipt should be sent. For other payment methods, please include a purchase order number from your institution with your order. For payment of international invoices by wire transfer, our preferred method of payment is through peerTransfer. With peerTransfer, you can: