Register | Log In
Welcome
Antigen list
Antibody list
New products
Order forms
Price list
My Account
collections
Antibody list > 

Cart Summary
Your Cart is Empty
View Cart
Information

Frequently asked questions

Contributor list

Contribute antibodies

Conference sponsorship

Board of advisors

Mission statement

Links of interest


Search
 
Advanced Search
 
210-287-3
$40.00


View product citations for antibody 210-287-3 on CiteAb
 
Required FieldProduct Form   
 
Tell a Friend

Antigen: Fimbrin

Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum Depositor: Gerisch, G.
Antigen Sequence: Depositors Institution: Max-Planck-Institut fuer Biochemie
Antigen Molecular Weight: 67 kDa Human Protein Atlas: Link
Positive Tested Species Reactivity: Dictyostelium Depositors Notes: The antibody was positively tested for Immunohistology.
Predicted Species Reactivity:   Gene:
Immunogen: Fimbrin, p67 extracted from contracted actin-myosin complex from starved cells Alternate Gene Names:
Immunogen Sequence: Isotype: MIgG1
Alternate Antibody Name: Clonality: Monoclonal
Alternate Antigen Name: Epitope Mapped: No
Myeloma Strain: PA1 Epitope Location or Sequence:
Uniprot ID: Host Species: mouse
Entrez Gene ID: Additional Characterization: Link
Antibody Registry ID: Additional Information: csA strain Ax2.  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
   
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
210-287-3 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 210-287-3)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ChIP References
  • ELISA References
  • FFPE References
  • FB References
  • FACS References
  • Microarray References
  • Epitope Map References
  • Gel Supershift References
  • All References

Initial Publication

IHC References

IP References

IF References

WB References

ChIP References

ELISA References

FFPE References

FB References

FACS References

Microarray References

Epitope Map References

Gel Supershift References

All References

Developmental Studies Hybridoma Bank
University of Iowa, Department of Biology
028 Biology Building East
Iowa City, Iowa 52242-1324


Phone: 319-335-3826
Secure Fax: 319-359-4079
Email: dshb@uiowa.edu


DSHB Webmaster
Copyright © 2015. All rights reserved.