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Antigen: Frizzled2

Hybridoma Cells Available: Yes
Antigen Species: Drosophila Depositor: Nusse, R.
Antigen Sequence: Depositors Institution: Department of Developmental Biology/HHMI
Antigen Molecular Weight: Human Protein Atlas: Link
Alternate Antigen Name: Depositors Notes:
Gene: Immunogen: a fusion protein expressed in E. coli
Alternate Gene Names: Immunogen Sequence:
Isotype: MIgG2a, lambda light chain Alternate Antibody Name:
Clonality: Monoclonal Additional Information: The origin of the antibody is the cytoplasmic tail of fz2; the last 89 amino acids were fused to GST. Immunization used 30 µg/boost in 1997/1998.
Epitope Mapped: No Myeloma Strain: Designation of SP2/0
Epitope Location or Sequence: Uniprot ID:
Host Species: mouse Entrez Gene ID:
Additional Characterization: Link Antibody Registry ID:
Positive Tested Species Reactivity: Drosophila Recommended Applications: Immunohistochemistry, Western Blot
References: Development 126, 577-586.  
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
12A7 was deposited to the DSHB by Nusse, R. (DSHB Hybridoma Product 12A7)
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.