Positive Tested Species Reactivity: All species labeled with BrdU
Depositors Notes: This antibody detects BrdU incorporated into cell nuclei during S-phase. 2N HCL treatment of nuclei increases antibody access to incorporated BrdU within the DNA (see Foster et al., 1987 for details).
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
G3G4 (anti-BrdU) was deposited to the DSHB by Kaufman, S.J. (DSHB Hybridoma Product G3G4 (anti-BrdU))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.