Depositors Notes: Doesn't recognize rat, hamster or primate antigen. GL2A7 Labels presumptive lysosomes (and late endosomes). For best immunochemistry results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.
Predicted Species Reactivity:
Immunogen: Mouse lysosomes (2% glutaraldehyde fixed) isolated by Percoll gradient fractionation of liver homogenate
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GL2A7 was deposited to the DSHB by Granger, B.L. (DSHB Hybridoma Product GL2A7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.