Depositors Notes: Does not cross react with mouse. Does not block iC3B rosetting. Binds to granulocytes, monocytes, macrophages, CD5+ B cells, hairy cell leukemia cells. Doesn't work well for western blotting.
Predicted Species Reactivity:
Immunogen: CD11b immunoprecipitated from Human granulocyte lysates + boost with Human live granulocytes.
Alternate Gene Names: MAC-1, MO1A, CR3
Immunogen Sequence: Full length protein
Alternate Antibody Name: LM2/1
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag188.8.131.52
Epitope Location or Sequence: I domain (aa 148-332)
Additional Information: Mac-1 is a macrophage differenciation antigen and a marker for microglia.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LM2/1.6.11 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product LM2/1.6.11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.