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191ME1
$40.00
 
  
 
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Antigen: Mutanase G

Hybridoma Cells Available: No
Antigen Species: Trichoderma harzianum Depositor: Gmuer, R.
Antigen Sequence: Depositors Institution: Institute of Oral Biology, ZZMK, University of Zuerich
Antigen Molecular Weight: Human Protein Atlas: Link
Predicted Species Reactivity:   Depositors Notes:
Gene: Immunogen: Mutanase, purified enzyme Mutanase G
Alternate Gene Names: Immunogen Sequence:
Isotype: MIgG1 Alternate Antibody Name:
Clonality: Monoclonal Alternate Antigen Name:
Epitope Mapped: No Myeloma Strain: Designation of F0
Epitope Location or Sequence: Uniprot ID:
Host Species: mouse Entrez Gene ID:
Additional Characterization: Link Antibody Registry ID:
Additional Information: *Cell lines will be distributed only with written consent from the director of the Institute of Oral Biology, University of Zuerich, Zuerich, Switzerland. Contact the DSHB for details (dshb@uiowa.edu). Immunization on 5 May, 2 June and 20 June 1986, used 110 micrograms of protein through an intramuscular subcutaneous route and incomplete freund's djuvant (50/50 v/v).  
Positive Tested Species Reactivity: Fungi Recommended Applications: Western Blot
   
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
191ME1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 191ME1)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored either at -20°C or -80°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 μg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 μg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

Initial Publication

IHC References

IP References

IF References

WB References

ChIP References

ELISA References

FFPE References

FB References

FACS References

Microarray References

Epitope Map References

Gel Supershift References

All References