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PCRP-POLE3-4E1
$40.00


View product citations for antibody PCRP-POLE3-4E1 on CiteAb
 
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Antigen: POLE3; Polymerase (DNA directed), epsilon 3, accessory subunit

Hybridoma Cells Available: Yes
Antigen Species: Human Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Antigen Sequence: Depositors Institution: National Institutes of Health
Antigen Molecular Weight: 16.86 Human Protein Atlas: Link
Positive Tested Species Reactivity: Human Depositors Notes:
Predicted Species Reactivity: Human, Mouse, Rat, Xenopus Gene: POLE3
Immunogen: Recombinant protein Alternate Gene Names: CHARAC17, CHRAC17, p17, Ybl1
Immunogen Sequence: Full length Isotype: MIgG1
Alternate Antibody Name: Clonality: Monoclonal
Alternate Antigen Name: Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14 Epitope Location or Sequence:
Uniprot ID: Host Species: mouse
Entrez Gene ID: Additional Characterization: Link
Antibody Registry ID: Additional Information: This antibody has been characterized by the NIH Protein Capture Reagents Program  
Recommended Applications: Immunoprecipitation, Microarray
   
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-POLE3-4E1 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-POLE3-4E1)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ChIP References
  • ELISA References
  • FFPE References
  • FB References
  • FACS References
  • Microarray References
  • Epitope Map References
  • Gel Supershift References
  • All References

Initial Publication

IHC References

IP References

IF References

WB References

ChIP References

ELISA References

FFPE References

FB References

FACS References

Microarray References

Epitope Map References

Gel Supershift References

All References

Developmental Studies Hybridoma Bank
University of Iowa, Department of Biology
028 Biology Building East
Iowa City, Iowa 52242-1324


Phone: 319-335-3826
Secure Fax: 319-359-4079
Email: dshb@uiowa.edu


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