Antigen: CD18 (integrin beta-2; beta subunit of CD11a, b, c), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Springer, T.A.
Host Species: mouse
Depositors Institution: Center for Blood Research
Positive Tested Species Reactivity: Human
Depositors Notes: Does not cross react with mouse. Binds to all leukocytes. Blocks CTL killing, ADCC killing of NK & granulocytes, homotypic B cell adhesion, gammaINF adhesion of monocytes, leukocyte-epithelial cell interactions. Doesn't appear to work for western blotting.
Additional Information: CD18 is a general marker of leukocytes.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TS1/22.214.171.124.4 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product TS1/126.96.36.199.4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.