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Antigen: Us9 (pseudorabies virus)

Hybridoma Cells Available: Yes
Antigen Species: pseudorabies virus Depositor: Enquist, L. / Lyman, M.
Antigen Sequence: Depositors Institution: Dept. of Molecular Biology, Princeton University
Antigen Molecular Weight: 17-20 kDa Human Protein Atlas: Link
Predicted Species Reactivity:   Depositors Notes: The antibody is a type II membane protein which is essential for axonal sorting of PRV structural components.
Gene: Immunogen: glutathione S-transferase-Us9 fusion protein
Alternate Gene Names: Immunogen Sequence:
Isotype: MIgG1 Alternate Antibody Name:
Clonality: Monoclonal Alternate Antigen Name:
Epitope Mapped: No Myeloma Strain:
Epitope Location or Sequence: Uniprot ID:
Host Species: mouse Entrez Gene ID:
Additional Characterization: Link Antibody Registry ID:
Additional Information: The origin of the immunogen is expressed in Escheriachia coli DH5 alpha cells and isolated as inclusion bodies. Works with pseudorabies virus  
Positive Tested Species Reactivity: Virus Recommended Applications: Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1A8 was deposited to the DSHB by Enquist, L. / Lyman, M. (DSHB Hybridoma Product 1A8)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored either at -20°C or -80°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

Initial Publication

IHC References

IP References

IF References

WB References

ChIP References

ELISA References

FFPE References

FB References

FACS References

Microarray References

Epitope Map References

Gel Supershift References

All References