Depositors Notes: This antibody works well for immunocytochemistry in embryos, imaginal discs and cultured cells, immunoprecipitation and Western blotting. Freezing significantly reduces the effective titre – supernatants are stable for a minimum of 5 years at 4° C with no loss of titre.
Predicted Species Reactivity:
Immunogen: Recombinant GST/Yan (aa 69-388) fusion protein
Additional Information: The Yan protein is also known as Ets DNA-binding protein pokkuri and anterior open.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-Yan 8B12H9 was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product anti-Yan 8B12H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.