Depositors Institution: University of Pennsylvania
Positive Tested Species Reactivity: Bovine, Chicken, Electrophorus, Fish, Human, Mouse, Rat, Zebrafish
Depositors Notes: acetylcholine binding (alpha) subunits of muscle nicotinic receptors, structural subunits of one subtype of neuronal nicotinic receptors from chicken brain; muscle nicotinic receptors of most species, receptors; immunohistology: from chicken brain; binds to the main immunogenic region on the extracellular surface of alpha subunits of muscle nicotinic receptors; binds to muscle nicotinic receptors from Electrophorus, Torpedo, Rana, chickens, rats, mice, humans, etc., but not to Xenopus. Binds to some neuronal nicotinic receptor subtypes in chickens and finches and cattle, but not to other subtypes in chickens, rats, cattle, and humans; Muscle nicotinic receptors are composed of 4 kinds of subunits with the stoichiometry alpha(2)beta gamma delta., Sites that bind acetylcholine and alpha-bungarotoxin are on the alpha subunits. Neuronal; nicotinic receptors are composed of 2 kinds of subunits, acetylcholine-binding subunits and structural subunits. There are several subtypes of neuronal nicotinic receptors, but none bind alpha-bungarotoxin.; mAb 35 has high affinity for native receptors; mAb 35 has very low affinity for denatured receptors and not good for blotting; mAb 35 can be used for affinity purification; cDNA sequences for the subunits of nicotinic receptors f(more...)
Recommended Applications: Function Blocking, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 35 was deposited to the DSHB by Lindstrom, J. (DSHB Hybridoma Product mAb 35)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.