Positive Tested Species Reactivity: Planaria, Schmidtea mediterranea
Depositors Notes: Strongly stains the regenerating blastema. This antibody labels a subset of unidentified cell nuclei distributed throughout the parenchyma (mesenchyme) with a concentration of positive nuclei near the cephalic ganglia and ventral nerve cords; a subset of labeled cells is gamma irradiation-sensitive.
Predicted Species Reactivity:
Immunogen: Formaldehyde-fixed cells from regenerating blastema of C1W4 clonal lineage
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D9-E11 was deposited to the DSHB by Zayas, R.M. (DSHB Hybridoma Product 1D9-E11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.