Cart Summary
Your Cart is Empty
View Cart

Frequently asked questions

Contributor list

Contribute antibodies

Conference sponsorship

Board of advisors

Mission statement

Links of interest

Advanced Search
Tell a Friend

Antigen: cardioactive peptide B

Hybridoma Cells Available: Yes
Antigen Species: Tritonia diomedea Depositor: Kempf, S.C.
Antigen Sequence: MET:ASN:TYR:LEU:ALA:PHE:PRO:ARG:MET Depositors Institution: Auburn University
Antigen Molecular Weight: SCPb 1.1414 kDa Human Protein Atlas: Link
Predicted Species Reactivity:   Depositors Notes:
Gene: Immunogen: small cardioactive peptide B, SCPB: MET:ASN:TYR:LEU:ALA:PHE:PRO:ARG:MET
Alternate Gene Names: Immunogen Sequence:
Isotype: MIgG1 Alternate Antibody Name: SCPb and SCP
Clonality: Monoclonal Alternate Antigen Name:
Epitope Mapped: No Myeloma Strain: Designation of NS-1
Epitope Location or Sequence: Uniprot ID:
Host Species: mouse Entrez Gene ID:
Additional Characterization: Link Antibody Registry ID:
Additional Information: Immunization was performed in 1984 and 100 µg of peptide-thyroglobulin conjugate was used. The injection was initiated subcutaneous injections followed by alternating subcutaneous and intraperitoneal injections and used complete Freund’s for the first immunization, incomplete Freund’s for the remainder.  
Positive Tested Species Reactivity: Invertebrate Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
17.1G6 was deposited to the DSHB by Kempf, S.C. (DSHB Hybridoma Product 17.1G6)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored either at -20°C or -80°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

Initial Publication

IHC References

IP References

IF References

WB References

ChIP References

ELISA References

FFPE References

FB References

FACS References

Microarray References

Epitope Map References

Gel Supershift References

All References