Positive Tested Species Reactivity: Avian, Bovine, Canine, Chicken, Chondrichthyes, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus, Zebrafish
Depositors Notes: This antibody does not cross react with Type III collagen propeptide. This recognize pro-collagen Type I in intracellular vesicles prior to collagen N-terminal pro-peptide cleavage. This antibody does not stain the ECM.
Predicted Species Reactivity:
Immunogen: CNBr cleavage of pro-collagen type I from dermatosparactice sheep
Additional Information: The N-terminal propeptide extends from a.a 23-161.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SP1.D8 was deposited to the DSHB by Furthmayr, H. (DSHB Hybridoma Product SP1.D8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.