Depositors Institution: Cornell University Medical College
Positive Tested Species Reactivity: Chicken, Fish, Lamb, Mouse, Rat
Depositors Notes: The D76 monoclonal antibodies bind to desmin but recognize different epitopes in all types of muscle including vascular smooth muscle but is unreactive with embryonic cardiac myocytes. D76 also labels electric organs in fish and myoid cells in chick and fish thymus.
Additional Information: Isopentane-cooled frozen sections or Triton X-100 extracted cultured cells are recommended for immunostaining. RRID: AB_528196
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D76 was deposited to the DSHB by Fischman, D.A. (DSHB Hybridoma Product D76)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.