Antigen: fluorescein isothiocyanate-conjugated to keyhold limpet hemocyanin
Cells Available: Yes
Host Species: mouse
Antigen Species: not applicable
Contributor: Voss, E.W.
References: Fluorescein Hapten: An Immunological Probe by Edward W. Voss, CRC Press (1984). This reference serves as a good review of the anti- fluorescein system. Perhaps 100 journal articles were written over the course of 20 years with Edward W. Voss as the principal investigator. The entire amino acid sequence of the Fab fragment has been published and the structure of the antibody solved by crystallization to about 3.0 angstroms.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
fluorescein was deposited to the DSHB by Voss, E.W. (DSHB Hybridoma Product fluorescein)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 μg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 μg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.
Developmental Studies Hybridoma Bank
University of Iowa, Department of Biology
028 Biology Building East
Iowa City, Iowa 52242-1324