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GD1a-2b
$40.00


View product citations for antibody GD1a-2b on CiteAb
 
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Antigen: Ganglioside GD1a

Hybridoma Cells Available: No
Antigen Species: Bovine Depositor: Schnaar, R.L.
Isotype: MIgG2b Antigen Sequence:
Host Species: mouse Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Human, Mouse, Rat, Vertebrates Depositors Notes: GD1a-2b recognizes all vertebrate species. Paraformaldehyde fixation works well for immnostaining; avoid most detergents and organic solvents.
Antigen Molecular Weight: 1.84 kDa Human Protein Atlas: Link
Predicted Species Reactivity:   Gene:
Immunogen: KLH-conjugated bovine brain GD1a Alternate Gene Names:
Alternate Antibody Name: Clonality: Monoclonal
Alternate Antigen Name: Epitope Mapped: Yes
Myeloma Strain: P3x653 Epitope Location or Sequence: GD1a
Uniprot ID: Immunogen Sequence:
Entrez Gene ID: Additional Characterization: Link
Antibody Registry ID: AB_2619569 Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. GD1a-2b cross-react with GT1aalpha [PMIDs: 11878808 12390975]. Western blot references include references for TLC immunooverlay.  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
   
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GD1a-2b was deposited to the DSHB by Schnaar, R.L. (DSHB Hybridoma Product GD1a-2b)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ChIP References
  • ELISA References
  • FFPE References
  • FB References
  • FACS References
  • Microarray References
  • Epitope Map References
  • Gel Supershift References
  • All References

Initial Publication

IHC References

IP References

IF References

WB References

ChIP References

ELISA References

FFPE References

FB References

FACS References

Microarray References

Epitope Map References

Gel Supershift References

All References

Developmental Studies Hybridoma Bank
University of Iowa, Department of Biology
028 Biology Building East
Iowa City, Iowa 52242-1324


Phone: 319-335-3826
Secure Fax: 319-359-4079
Email: dshb@uiowa.edu


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