Depositors Notes: 9B2.1 Ab recognizes the Drosophila protein glass. Glass is expressed at high levels in the nucleus of the developing photoreceptor cells in a sequence that reflects their acquisition of neuronal identity. 9B2.1 gives strong staining of the morphogenetic furrow and all of the cells that arise from the furrow.
Predicted Species Reactivity:
Immunogen: Glass-trp E fusion protein (see PMID: 2010085)
Additional Information: RRID: AB_528268. FBgn0004618. Use periodate-lysine-paraformaldehyde (PLP) fix only.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9B2.1 anti-glass was deposited to the DSHB by Rubin, G.M. (DSHB Hybridoma Product 9B2.1 anti-glass)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.