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1B2 JW
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Antigen: hexokinase (Type I isozyme)

Hybridoma Cells Available: Yes
Antigen Species: rat Depositor: Wilson, J.E.
Antigen Sequence: Depositors Institution: 301 Biochemistry, Dept. of Biochemistry & Molecular Biology, Michigan State University
Antigen Molecular Weight: Human Protein Atlas: Link
Additional Information: The origin of the antibody is purified from rat brain. You can find details of the immunization and subsequent fusion protocol are found in Finney et al. (1984) J.
Biol. Chem. 259: 8232-8237. Immunization of 100 micrograms of purified rat type I hexokinase was injected intraperitoneally and the adjuvant is freunds.
Depositors Notes: The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. Binding of the antibody does not affect the kinetic properties of the rat Type I isozyme [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178]. The epitope recognized is a ÒconformationalÓ (or ÒdiscontinuousÓ) epitope, and immunoreactivity is affected by conformational changes in the Type I hexokinase, e.g., as a result of binding certain ligands [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178]. rat; crossreactivity with Type I isozyme from other mammalian species has not been investigated. Monoclonal 1B2 recognizes an epitope in the C-terminal half of rat Type I hexokinase. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely. Crossreactivity with the Type II or Type III isozymes is thought to be unlikely.
Gene: Immunogen: Type I isozyme of rat hexokinase
Alternate Gene Names: Immunogen Sequence:
Isotype: MIgG2b Alternate Antibody Name:
Clonality: Monoclonal Alternate Antigen Name:
Epitope Mapped: No Myeloma Strain: Designation: Sp2/O-Ag14
Epitope Location or Sequence: Uniprot ID:
Host Species: mouse Entrez Gene ID:
Additional Characterization: Link Antibody Registry ID:
Positive Tested Species Reactivity: Rat Recommended Applications: ELISA, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1B2 JW was deposited to the DSHB by Wilson, J.E. (DSHB Hybridoma Product 1B2 JW)
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

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