Depositors Institution: Fred Hutchison Cancer Research Center
Positive Tested Species Reactivity: Human
Depositors Notes: P4C10 inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. Fixation for immunostaining: formalin, 4% paraformaldehyde, acetone-methanol, ice-cold methanol. The epitope recognized by P4C10 is not trypsin sensitive. This antibody doesn't appear to work for western blotting as it recognizes the native protein.
Additional Information: While this antibody has been reported not to work for western blotting by the hybridoma contributor (Wayner, E.A.), Williams et al. have reported using it succesfully [PMID: 24807903]. This antibody will recognize all known isoforms.
Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4C10 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P4C10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.