Positive Tested Species Reactivity: Human, Primate
Depositors Notes: 7H2 reacts with alphaIIbbeta3 complex on platelets and has variable effects on platelet function. The epitope recognized by 7H2 is located on the beta3 PSI domain (unpublished mutational analysis).
Predicted Species Reactivity:
Gene: ITGB3
Immunogen: Human platelets
Alternate Gene Names: GP3A, CD61
Immunogen Sequence: Full length protein
Isotype: MIgG1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63-Ag8.653
Epitope Location or Sequence: integrin beta3 PSI domain
Additional Information: 7H2 recognizes free beta3 as weel as alphaVbeta3 and alphaIIbbeta3 and their pro-forms. In immunoblots, 7H2 doesn't recognize its target under reducing conditions.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7H2 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 7H2)
Storage and Handling Recommendations
Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.
