Antigen: myosin heavy chain, fast, extraocular specific (MYH13)
Hybridoma Cells Available: No
Antigen Species: cat
Depositor: Lucas, C.
Isotype: MIgM, kappa light chain
Host Species: mouse
Depositors Institution: University of Sydney
Positive Tested Species Reactivity: Cat, Human, Rabbit, Rat
Depositors Notes: 4A6 reacts extraocular specific fast myosin heavy chain from rabbit extraocular muscles by western blot, and with extraocular specific fast muscle fibres on rabbit, rat and cat extraocular and laryngeal muscles by immunohistochemistry.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4A6 was deposited to the DSHB by Lucas, C. (DSHB Hybridoma Product 4A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.