Additional Information: See Georgadaki and Zagris (2014) Research Journal of Developmental Biology Vol. 1 Article 3. Chmical properties can be found See Rauch et al., 1991, 1992; Meyer-Puttlitz et al., 1995, 1996; Margolis et al., 1996; Margolis and Margolis, 1997. RRID:AB_2149837
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1F6 was deposited to the DSHB by Margolis, R.K. / Margolis, R.U. (DSHB Hybridoma Product 1F6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.