Positive Tested Species Reactivity: Acorn worm, Bovine, Canine, Human, Monkey, Pig, Rabbit, Rat
Depositors Notes: The isotype of this monoclonal antibody was originally reported as IgG3 (PMID: 2476206). This antibody recognizes osteonectin (SPARC)-containing cartilages in invertebrate species. See PMID: 16351944.
Predicted Species Reactivity:
Immunogen: Purified bovine osteonectin (SPARC)
Alternate Gene Names:
Immunogen Sequence: Full length protein
Isotype: MIgG1, kappa light chain
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: x63-AG8.653
Epitope Location or Sequence: The epitope bound by AON-1 is cryptic and often requires tissue pretreatment for exposure. See PMID: 19435834 & 2476206.
Recommended Applications: ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AON-1 was deposited to the DSHB by Termine, J.D. (DSHB Hybridoma Product AON-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.