Depositors Notes: This antibody recognizes poly(GP) dipeptide repeat proteins produced from an expanded hexanucleotide (GGGGCC) repeat in the first intron of C9ORF72, the most common known genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The expanded GGGGCC repeat undergoes an atypical form of translation - repeat-associated non-ATG (RAN) translation - forming various dipeptide repeat proteins, one of which is poly(GP). The hybridoma was commercially generated with funds from the Target ALS Foundation.
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
poly(GP) TALS 828.66 was deposited to the DSHB by Target ALS Foundation (DSHB Hybridoma Product poly(GP) TALS 828.66)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.