Depositors Notes: This mAb differs from other anti-DPTP10D, as it recognizes the native form of DPTP10D and works well for IP. this mAb works much better than 45E10 (a different anti-DPTP10D clone available from DSHB) for staining larval material; the two mAbs are equivalent on embryos.
Predicted Species Reactivity:
Immunogen: Recombinant fusion protein containing the extracellular domain of PTP10D produced in monkey COS7 cells.
Alternate Gene Names: CG1817
Immunogen Sequence: Partial protein
Isotype: MIgG1, kappa light chain
Alternate Antibody Name: 8B2
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: HL-1
Epitope Location or Sequence: Extracellular domain
Additional Information: PTP10D is a receptor tyrosine phosphatase on motor neurons. 8B22F5stains the tracheal system and the axons of the central nervous system. The specificity of 8B22F5 was tested on Ptp10D mutants by immunoprecipitation and immunohistology.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8B22F5 was deposited to the DSHB by Zinn, K. (DSHB Hybridoma Product 8B22F5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.