Depositors Institution: Department of Biochemistry & Microbiology, University of Victoria
Positive Tested Species Reactivity: Echinoderm, Invertebrate, Starfish
Depositors Notes: Immunostaining fixation is used ice cold methanol, 4% paraformaldehyde (not longer than 15 minutes). The characterization of the antigen was Mass Spec immunoprecipitated material identify Sp-SynB, mammalian cells expressing SpSynB are immunoreactive, antibodies produced against Sp-SynB have identical patterns ofimmunoreactivity, Burke et al. 2006. Also works with deuterostomes, ascidian, echinderms, hemichordates.
Additional Information: We recommend addition of glycerol to 50% and storage at -20C for long term storage. RRID:AB_2617214
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1E11 was deposited to the DSHB by Burke, R.D. (DSHB Hybridoma Product 1E11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.