Positive Tested Species Reactivity: Chicken, Fish, Human, Mouse, Rat, Xenopus
Depositors Notes: mAB 30 (asv30) as a lower affinity than mAb 48 (asv 48). The two antibodies recognize the same antigen, Syt1. mAB 30 (asv30) is detergent sensitive: TritonX-100 sensitive epitope (reversible), epitope present in presence of CHAPS.
Predicted Species Reactivity:
Immunogen: Synaptic junctional complex proteins prepared from rat brain synaptic membranes
Alternate Gene Names: P65
Immunogen Sequence: Full length protein
Isotype: MIgG2a, kappa light chain
Alternate Antibody Name: SV 30
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Cytoplasmic (aa 80-421)
Additional Information: Not available to for-profit organizations. For further information please contact the Office of Innovation, Technology, and Alliances at the University of California. Refer to case number 95-082. Email : firstname.lastname@example.org Telephone: 415 502-1603. Researchers at non-profit institutions must sign an additional compliance form prior to purchasing this antibody. The compliance form is available by emailing email@example.com or by calling 319 335-3826.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB 30 (asv30) was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product mAB 30 (asv30))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.