Positive Tested Species Reactivity: Chicken, Fish, Human, Lamprey, Mouse, Rat, Xenopus, Zebrafish
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Predicted Species Reactivity:
Immunogen: Synaptic junctional complex proteins prepared from rat brain synaptic membranes
Alternate Gene Names: P65
Immunogen Sequence: Full length protein
Isotype: MIgG2b, kappa light chain
Alternate Antibody Name: SV 48
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Cytoplasmic (aa 80-421)
Additional Information: This antibody was validated for tissue fixed with 4% paraformaldehyde and cryosections following OCT embedding. Detergent sensitive / Triton X-100. This antibody recognizes synaptotagmin 1 and does not cross react with synaptotagmin 2. See Fox and Sanes (2007, PUDMED 17492637). mAb 48 (asv 48) has a higher affinity than mAb 30 (asv30). The two antibodies recognize the same antigen, Syt1.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 48 (asv 48) was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product mAb 48 (asv 48))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.