Depositors Institution: Department of Physiology, University of California
Positive Tested Species Reactivity: Chicken, Fish, Human, Lamprey, Mouse, Rat, Xenopus, Zebrafish
Depositors Notes: Not available to for-profit organizations. For further information please contact the Office of Innovation, Technology, and Alliances at the University of California. Refer to case number 95-082. Email : firstname.lastname@example.org Telephone: 415 502-1603. Researchers at non-profit institutions must sign an additional compliance form prior to purchasing this antibody. The compliance form is available by emailing email@example.com or by calling 319 335-3826.
Additional Information: This antibody was validated for tissue fixed with 4% paraformaldehyde and cryosections following OCT embedding. Detergent sensitive / Triton X-100. This antibody recognizes synaptotagmin 1 and does not cross react with synaptotagmin 2. See Fox and Sanes (2007, PUDMED 17492637). mAb 48 (asv 48) has a higher affinity than mAb 30 (asv30). The two antibodies recognize the same antigen, Syt1.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 48 (asv 48) was deposited to the DSHB by Reichardt, L. (DSHB Hybridoma Product mAb 48 (asv 48))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.