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169-477-5
$40.00
 
  
 
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Antigen: talin

Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium discoideum Depositor: Gerisch, G.
Antigen Sequence: Depositors Institution: MPI for Biochemistry, Cell-Dynamics
Antigen Molecular Weight: 205 kD Human Protein Atlas: Link
Predicted Species Reactivity:   Depositors Notes:
Gene: Immunogen: Talin, C-terminus, purified talin from actin-affinity column elute
Alternate Gene Names: Immunogen Sequence:
Isotype: MIgG1 Alternate Antibody Name:
Clonality: Monoclonal Alternate Antigen Name:
Epitope Mapped: No Myeloma Strain: PAIB3Ag8I
Epitope Location or Sequence: Uniprot ID:
Host Species: mouse Entrez Gene ID:
Additional Characterization: Link Antibody Registry ID:
Additional Information: The adjuvant used in immunization was alugel S. The origin of the antibody was Dictyostelium discoideum.  
Positive Tested Species Reactivity: Dictyostelium Recommended Applications: Immunohistochemistry, Western Blot
   
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
169-477-5 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 169-477-5)

Storage and Handling Recommendations Usage Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored either at -20°C or -80°C. While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 μg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 μg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.  

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