tor23

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DSHB Data Sheet

Catalog Fields

Product Name/ID: tor23
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ache
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2722484 
Uniprot ID: P07692 
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Purified synaptosomes from Torpedo electric organ
Clone:
Immunogen Sequence:
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: Acetylcholinesterase, presynaptic
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/20/2017
Antigen Molecular Weight: 180 kDa, 66.7 kDa
Depositor: Harland, Richard
Antigen Sequence:
Depositor Institution: University of California, Berkeley
Antigen Species: Torpedo
Depositor Notes: This antibody stains spinal cord, cerebral cortex and pyramidal cells in the hippocampal CA2 region. The antigen recognized by Tor23 is also expressed in hNTERA-2 teratocarcinoma.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Rat, Torpedo, Xenopus
Additional Information: This antibody was generated by Pinky Kushner in 1984 as part of a library of monoclonal antibodies to Torpedo cholinergic synaptosomes. RRID:AB_2722484
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
tor23 was deposited to the DSHB by Harland, Richard (DSHB Hybridoma Product tor23)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    A library of monoclonal antibodies to Torpedo cholinergic synaptosomes.
    Kushner PD
    Journal of neurochemistry 43.3 (1984 Sep): 775-86.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    IF References

    Torpedo monoclonal antibodies react with components of the human peripheral nervous system.
    Kushner PD
    Muscle & nerve 11.1 (1988 Jan): 10-20.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    A human teratocarcinoma which expresses a rare neuronal cell surface antigen.
    Woloshin PI
    Brain research 388.3 (1987 Sep): 271-5.

    An atlas of a rare neuronal surface antigen in the rat central nervous system.
    Kushner PD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.8 (1988 Aug): 3035-56.

    Monoclonal antibody Tor 23 binds a subset of neural cells in the human cortex and displays an altered binding distribution in Alzheimer's disease.
    Greco CM
    Journal of neuropathology and experimental neurology 48.6 (1989 Nov): 692-708.

    Family of human neuronal external surface epitopes defined by Torpedo monoclonal antibodies.
    Kushner PD
    Journal of neuroscience research 25.4 (1990 Apr): 486-502.

    WB References

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    Monoclonal antibody Tor 23 binds a subset of neural cells in the human cortex and displays an altered binding distribution in Alzheimer's disease.
    Greco CM
    Journal of neuropathology and experimental neurology 48.6 (1989 Nov): 692-708.

    Family of human neuronal external surface epitopes defined by Torpedo monoclonal antibodies.
    Kushner PD
    Journal of neuroscience research 25.4 (1990 Apr): 486-502.

    IHC References

    Family of human neuronal external surface epitopes defined by Torpedo monoclonal antibodies.
    Kushner PD
    Journal of neuroscience research 25.4 (1990 Apr): 486-502.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    An atlas of a rare neuronal surface antigen in the rat central nervous system.
    Kushner PD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.8 (1988 Aug): 3035-56.

    IP References

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    ELISA References
    All References

    Family of human neuronal external surface epitopes defined by Torpedo monoclonal antibodies.
    Kushner PD
    Journal of neuroscience research 25.4 (1990 Apr): 486-502.

    Monoclonal antibody Tor 23 recognizes a determinant of a presynaptic acetylcholinesterase.
    Weber R
    Journal of neurochemistry 48.6 (1987 Jun): 1942-53.

    A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane.
    Kelly RB
    Proceedings of the National Academy of Sciences of the United States of America 80.23 (1983 Dec): 7342-6.

    An atlas of a rare neuronal surface antigen in the rat central nervous system.
    Kushner PD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.8 (1988 Aug): 3035-56.

    Torpedo monoclonal antibodies react with components of the human peripheral nervous system.
    Kushner PD
    Muscle & nerve 11.1 (1988 Jan): 10-20.

    A human teratocarcinoma which expresses a rare neuronal cell surface antigen.
    Woloshin PI
    Brain research 388.3 (1987 Sep): 271-5.

    Monoclonal antibody Tor 23 binds a subset of neural cells in the human cortex and displays an altered binding distribution in Alzheimer's disease.
    Greco CM
    Journal of neuropathology and experimental neurology 48.6 (1989 Nov): 692-708.

    A library of monoclonal antibodies to Torpedo cholinergic synaptosomes.
    Kushner PD
    Journal of neurochemistry 43.3 (1984 Sep): 775-86.

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