Antigen: Vasa ATP-dependent RNA helicase, isoform A
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Jan, Y.-N.
Host Species: mouse
Depositors Institution: University of California San Francisco
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Mab46F11 was generated using horseradish peroxidase as the immunogen. Mab46F11 does not recognize HRP and the relationship of HRP to the Vasa antigen is not known. The antigen is associated with polar granules and nuclear bodies during Drosophila embryogenesis.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Vasa 46F11 was deposited to the DSHB by Jan, Y.-N. (DSHB Hybridoma Product Vasa 46F11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable.
To ensure retention of antibody activity, we recommend aliquotting the product into two parts:
1) a volume of antibody stored at 4°C to be used within two weeks.
2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC),
immunofluorescence(IF) and staining is 2-5 µg/ml.
For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. See our FAQ section for additional information.