10D9G11
Catalog Fields
Antigen: Germ cell nuclear antigen 1; GCNA1
Available to For-Profits: Yes
Antigen Species: Mouse
Hybridoma Cells Available (Non-Profit): No
Isotype: RIgM
Depositor: Enders, George
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Mouse, Rat
Depositors Institution: University of Kansas Medical School
Antigen Molecular Weight: 80-110 kDa depending upon modification
Depositors Notes: Over 100 publications have used this antibody to recognize germ cells. The protein target has recently be identified as the Gcna gene product. See PMID: 27718356
Immunogen: Pachytene spermatocytes
Gene: Gcna
Alternate Antibody Name:
Alternate Gene Names: Gcna1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/N
Epitope Mapped: No
Entrez Gene ID: 107425
Immunogen Sequence:
Antibody Registry ID: AB_2629436
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: The epitope is sensitive to glutarldehyde and trypsin. RRID:AB_2629436
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
10D9G11 was deposited to the DSHB by Enders, George (DSHB Hybridoma Product 10D9G11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Developmentally regulated expression of a mouse germ cell nuclear antigen examined from embryonic day 11 to adult in male and female mice.
May JJ 2nd
Developmental biology 163.2 (1994 Jun): 331-40.