11C1.3

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SKU: 11C1.3-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 11C1.3
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: FBN1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2794754 
Uniprot ID: P98133 
RRID:  
Entrez Gene ID: 281154 
Clonality: Monoclonal
Immunogen: Ocular zonular microfibrils
Clone:
Immunogen Sequence: Full length
Myeloma Strain: P3 X 63A8.653
Epitope Mapped: No
Antigen Name: Fibrillin-1
Epitope Location or Sequence: a.a. 723-909
Alternate Antigen Name:
Deposit Date: 9/21/2023
Antigen Molecular Weight: 312.2 kDa
Depositor: UAB Research Foundation
Antigen Sequence:
Depositor Institution: U of Alabama, Birmingham
Antigen Species: Bovine
Depositor Notes: Hybridoma developed by R. Mayne.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Bovine, Human, Japanese Macaque
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
11C1.3 was deposited to the DSHB by UAB Research Foundation (DSHB Hybridoma Product 11C1.3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Immunization with undenatured bovine zonular fibrils results in monoclonal antibodies to fibrillin.
    Mayne R
    Matrix biology : journal of the International Society for Matrix Biology 14.1 (1994 Jan): 41-9.

    IF References

    Immunization with undenatured bovine zonular fibrils results in monoclonal antibodies to fibrillin.
    Mayne R
    Matrix biology : journal of the International Society for Matrix Biology 14.1 (1994 Jan): 41-9.

    WB References

    Immunization with undenatured bovine zonular fibrils results in monoclonal antibodies to fibrillin.
    Mayne R
    Matrix biology : journal of the International Society for Matrix Biology 14.1 (1994 Jan): 41-9.

    IHC References

    Immunization with undenatured bovine zonular fibrils results in monoclonal antibodies to fibrillin.
    Mayne R
    Matrix biology : journal of the International Society for Matrix Biology 14.1 (1994 Jan): 41-9.

    All References

    Immunization with undenatured bovine zonular fibrils results in monoclonal antibodies to fibrillin.
    Mayne R
    Matrix biology : journal of the International Society for Matrix Biology 14.1 (1994 Jan): 41-9.

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