138AA1.1

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SKU: 138AA1.1-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 138AA1.1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_2618047 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Aggregatibacter actinomycetemcomitans, strain OMZ 247 (= NCTC 9710 or ATCC 33384), cell-free culture supernatant of 24 h culture in fluid medium
Clone:
Immunogen Sequence:
Myeloma Strain: Designation of F0
Epitope Mapped: No
Antigen Name: Aggregatibacter actinomycetemcomitans serotype c
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/1/2011
Antigen Molecular Weight:
Depositor: Gmuer, R.
Antigen Sequence:
Depositor Institution: University of Zuerich, Institute of Oral Biology, ZZMK
Antigen Species: Aggregatibacter actinomycetemcomitans
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: gram-negative bacteria
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (dshb@uiowa.edu). Immunization took the intraperitoneal route and used AlK(SO4) precipitate of approximately 100 ml culture supernatant amount in 1986. THe adjuvant of the antibody during immunization was 50% incomplete Freund's adjuvant (1st immunization), none (2nd immunization). RRID:AB_2618047
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
138AA1.1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 138AA1.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • All References
  • Initial Publication

    Monoclonal antibodies for the detection of 'periodontopathic' bacteria.
    Guggenheim B
    Archives of oral biology 35 Suppl. (1990): 145S-151S.

    All References

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