Antigen: Mutanase G
Depositor: Gmuer, R.
Antigen Species: Trichoderma harzianum
Depositors Institution: Institute of Oral Biology, ZZMK, University of Zuerich
Depositors Notes: Western blot Mab detects epitope D and present on band of approx. 42 kDa. The chemical properties of the antigen are an enzyme that degrades water insoluble _-(1->3)-linked glucan polysaccharide (so called Mutan) as produced by Streptococcus mutans and Streptococcus sobrinus.
Host Species: mouse
Positive Tested Species Reactivity: Fungi
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (email@example.com). csA from strain trichoderma harzianum OMZ 799. RRID:AB_2618055
Antigen Molecular Weight: 43 kDa
Immunogen: Mutanase, purified by HPLC
Myeloma Strain: F0
Antibody Registry ID: AB_2618055
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
200MH1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 200MH1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).