Antigen: Mutanase G
Hybridoma Cells Available: No
Antigen Species: Trichoderma harzianum
Depositor: Gmuer, R.
Host Species: mouse
Depositors Institution: Institute of Oral Biology, ZZMK, University of Zuerich
Positive Tested Species Reactivity: Fungi
Depositors Notes: Western blot Mab detects epitope D and present on band of approx. 42 kDa. The chemical properties of the antigen are an enzyme that degrades water insoluble _-(1->3)-linked glucan polysaccharide (so called Mutan) as produced by Streptococcus mutans and Streptococcus sobrinus.
Predicted Species Reactivity:
Immunogen: Mutanase, purified by HPLC
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: F0
Epitope Location or Sequence:
Antibody Registry ID: AB_2618055
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (firstname.lastname@example.org). csA from strain trichoderma harzianum OMZ 799. RRID:AB_2618055
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
200MH1 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 200MH1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.