21-8C6

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$40.00
SKU: 21-8C6-s
View product citations for antibody 21-8C6 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Benzodiazepines
Hybridoma Cells Available: No
Antigen Species: N.A.
Depositor: De Blas, Angel L.
Isotype: MIgG
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Connecticut
Positive Tested Species Reactivity: N.A.
Depositors Notes: This antibody has high affinity for diazepam, flunitrazepam, Ro5-4864, Ro5-3453, Ro11-6896 and Ro5-3438. This mAb is excellent from immunoassays for benzodiazepine drugs.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Benzodiazepine-BSA
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2877067 
Additional Information:
Recommended Applications: ELISA, FFPE, Function Blocking, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
21-8C6 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 21-8C6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    Monoclonal antibodies to benzodiazepines.
    Rayner CA
    Journal of neurochemistry 45.6 (1985 Dec): 1748-53.

    IP References

    Monoclonal antibodies to benzodiazepines.
    Rayner CA
    Journal of neurochemistry 45.6 (1985 Dec): 1748-53.

    ELISA References

    Monoclonal antibodies to benzodiazepines.
    Rayner CA
    Journal of neurochemistry 45.6 (1985 Dec): 1748-53.

    All References

    Monoclonal antibodies to benzodiazepines.
    Rayner CA
    Journal of neurochemistry 45.6 (1985 Dec): 1748-53.

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