23C7 anti-wit

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SKU: 23C7 anti-wit

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 23C7 anti-wit
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: WIT
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528513 
Uniprot ID: M9PE88 Q9VZI9 
RRID:  
Entrez Gene ID: 44096 
Clonality: Monoclonal
Immunogen: wit, c-term (aa 564-680)
Clone:
Immunogen Sequence: aa 564-680
Myeloma Strain: P3 x 63 Ag8.653
Epitope Mapped: No
Antigen Name: wit
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/14/2002
Antigen Molecular Weight: 903 aa, 100 kDa
Depositor: Goodman, C.
Antigen Sequence:
Depositor Institution: University of California, Berkeley, Department of Molecular and Cell Biology
Antigen Species: Drosophila
Depositor Notes: Functional effects Ser/Thr-kinase
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: RRID:AB_528513
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Function Blocking, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
23C7 anti-wit was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 23C7 anti-wit)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
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