23C7 anti-wit

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SKU: 23C7 anti-wit
View product citations for antibody 23C7 anti-wit on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: wit
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Depositors Institution: University of California, Berkeley, Department of Molecular and Cell Biology
Host Species: mouse
Depositors Notes: Functional effects Ser/Thr-kinase
Positive Tested Species Reactivity: Drosophila
Gene: WIT
Antigen Molecular Weight: 903 aa, 100 kDa
Clonality: Monoclonal
Immunogen: wit, c-term (aa 564-680)
Immunogen Sequence: aa 564-680
Myeloma Strain: P3 x 63 Ag8.653
Additional Information: RRID:AB_528513
Uniprot ID: M9PE88 Q9VZI9 
Entrez Gene ID: 44096 
Antibody Registry ID: AB_528513 
Recommended Applications: Function Blocking, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
23C7 anti-wit was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 23C7 anti-wit)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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