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SKU: 252-234-2
View product citations for antibody 252-234-2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: calreticulin, recombinant Dictyostelium
Hybridoma Cells Available: Yes
Antigen Species: Dictyostelium
Depositor: Gerisch, G.
Isotype: MIgG1
Depositors Institution: MPI for Biochemistry, Cell-Dynamics
Host Species: mouse
Gene: crtA
Positive Tested Species Reactivity: Dictyostelium
Clonality: Monoclonal
Antigen Molecular Weight: ca. 60 kDa
Epitope Mapped: No
Immunogen: calreticulin
Additional Information: Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle RRID:AB_2069810
Myeloma Strain: PAIB3Ag8I
Uniprot ID: Q23858 
Antibody Registry ID: AB_2069810 
Recommended Applications: Function Blocking, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
252-234-2 was deposited to the DSHB by Gerisch, G. (DSHB Hybridoma Product 252-234-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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