2B6

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$40.00
SKU: 2B6
View product citations for antibody 2B6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: hexokinase (Type I isozyme)
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Wilson, J.E.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Michigan State University, Dept. of Biochemistry & Molecular Biology
Positive Tested Species Reactivity: Rat
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: HK1
Immunogen: Type I isozyme of rat hexokinase
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: P05708 
Immunogen Sequence:
Entrez Gene ID: 25058 
Additional Characterization:  
Antibody Registry ID: AB_528281 
Additional Information: The antibody is reactive with the native enzyme (ELISA) but not the denatured form of the enzyme and thus is not useful for immunoblots. The functional effects of this antibody inhibits rat Type I hexokinase activity to >95%, the effect being on V max with no significant effect of Kms for either glucose or ATP [Smith and Wilson (1992) Arch. Biochem. Biophys. 292: 165-178. RRID:AB_528281
Recommended Applications: ELISA
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2B6 was deposited to the DSHB by Wilson, J.E. (DSHB Hybridoma Product 2B6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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